isocratic การใช้

Additionally, they were able to tune the retention factor for the analytes through isocratic temperature gradient elution.
In isocratic elution, peak width increases with retention time linearly according to the equation for N, the number of theoretical plates.
The separation took place under isocratic conditions using pure water as the mobile phase, and controlled the temperature using a water bath.
In isocratic elution, the selectivity does not change if the column dimensions ( length and inner diameter ) change that is, the peaks elute in the same order.
However, it is also possible to concentrate material from a dilute stream at the head of a chromatographic column and then switch conditions to elute the adsorbed material in conventional isocratic or gradient modes.