n-bromosuccinimide การใช้

There were 5 . 9 tip residues in cml . the purified cml was modified with n - bromosuccinimide ( nbs ) in hac - naac buffer containing 8mol / l urea or not
The change of agglutinating activity , cd spectrum and fls of lra in different temperature , ph and different chemicals indicated that lra had partial hemagglutinating activity at ph2 . 0 ( 50 % ) , a temperature above 100 ( 60 % ) and after modified by n - bromosuccinimide ( mbs ) , the activity lost completely , modified by depc , the lra had a little activity , the other groups modified such as arg , tyr , glu , asp did n ' t effect the hemagglutinating activity of lra . the result indicated that trp residues were essential to the hemagglutinating activity and were involved in carbohydrate - binding site