density gradient centrifugations การใช้

• Purification before culturing can be accomplished by CsCl density gradient centrifugation.
• Heavy liquids are often used for determination of density in mineralogy, for density gradient centrifugation and for separating mixtures.
• They used density gradient centrifugation to determine which isotope or isotopes of nitrogen were present in the DNA after cycles of replication.
• Sperm may be washed by density gradient centrifugation or by a " " direct swim-up " " technique that does not involve centrifugation.
• The "'buffy coat "'is the anticoagulated blood sample that contains most of the white blood cells and platelets following density gradient centrifugation of the blood.
• In more detailed studies of two SLE sera, sucrose density gradient centrifugation showed that greater than 50 % of the IgG binding to undigested Raji cells sedimented in the 7S region.
• Due to its very high solubility in water ( max . density 3.1 g / cm 3 ) SPT is widely used as a heavy liquid for gravity separation ( sink swim analysis ) and density gradient centrifugation.
• The term satellite DNA originates from the observation in the 1960s of a fraction of sheared DNA that showed a distinct buoyant density, detectable as a  satellite peak in density gradient centrifugation, and that was subsequently identified as large centromeric tandem repeats.
• The method, equilibrium density gradient centrifugation, was sufficiently sensitive that Meselson and Stahl were able to separate DNA containing the heavy isotope of nitrogen, 15 N, from DNA made of the lighter isotope, 14 N . In their classic experiment, described and analyzed in a book by science historian Frederic L . Holmes, they grew the bacterium " Escherichia coli " for many generations in medium containing 15 N as the only nitrogen source and then switched the bacteria to growth medium containing 14 N instead.