eosin y การใช้

• Eosin Y is then added to produce a " neutral " dye.
• Eosin Y is a tetrabromo derivative of fluorescein.
• Examples include eosin Y and rose bengal.
• However, the organic photoredox catalyst Eosin Y was used rather than a ruthenium or iridium complex.
• Giemsa improved the Romanowsky stain ( Eosin Y and Methylene Blue ) by stabilizing this dye solution with glycerol.
• Similar photoredox generation of iminium ions has furthermore been achieved using purely organic photoredox catalysts, such as Rose Bengal and Eosin Y.
• For staining, eosin Y is typically used in concentrations of 1 to 5 percent weight by volume, dissolved in water or ethanol.
• It is a critical component of Papanicolaou stains together with eosin Y and bismarck brown Y . It usually comes as a disodium salt.
• The nuclear staining is followed by counterstaining with an aqueous or alcoholic solution of eosin Y, which colors eosinophilic structures in various shades of red, pink and orange.
• Field's stain consists of two parts-Field's stain A is methylene blue and Azure 1 dissolved in phosphate buffer solution; Field's stain B is Eosin Y in buffer solution.
• If only synthetic Azure B and Eosin Y is used, it may serve as a standardized Giemsa stain; but, without methylene blue, the normal neutrophilic granules tend to overstain and look like toxic granules.
• Differences between Leishman's and Reuter's methods were : Leishman used methanol ( like Jenner ) and substituted Eosin B for Eosin Y, whereas Reuter used ethyl alcohol and rightly stressed the importance of using an absolutely pure solvent.
• This type of media uses the biochemical characteristics of a microorganism growing in the presence of specific nutrients or indicators ( such as neutral red, phenol red, eosin y, or methylene blue ) added to the medium to visibly indicate the defining characteristics of a microorganism.
• Paul Ehrlich in 1880 described the use of " neutral " dyes  mixtures of acidic and Dmitri Leonidovich Romanowsky independently developed techniques using a mixture of Eosin Y and modified methylene blue ( methylene azure ) that produced a surprising hue unattributable to either staining component : a shade of purple.
• The related stains are known as the "'buffered Wright stain "', the "'Wright-Giemsa stain "'( a combination of Wright and Giemsa stains ), and the "'buffered Wright-Giemsa stain "', and specific instructions depend on the solutions being used, which may include eosin Y, azure B, and methylene blue ( some commercial preparations combine solutions to simplify staining ).