glutamylcysteine synthetase การใช้

• Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
• The compound inhibits gamma-glutamylcysteine synthetase, the enzyme required in the first step of antiparasitic drugs.
• Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis.
• For example, the tripeptide glutathione is synthesized in two steps from free amino acids, by two enzymes : gamma-glutamylcysteine synthetase and glutathione synthetase.
• In contrast to ?-glutamylcysteine synthetase, glutathione synthetase accepts a large variety of glutamyl-modified analogs of ?-glutamylcysteine, but is much more specific for cysteine-modified analogs of ?-glutamylcysteine.
• The activity of GSTs is dependent upon a steady supply of GSH from the synthetic enzymes gamma-glutamylcysteine synthetase and glutathione synthetase, as well as the action of specific transporters to remove conjugates of GSH from the cell.
• In the first step, gamma-glutamylcysteine synthetase condenses cysteine and glutamic acid through a peptide bond formed between the side-chain carboxyl of the glutamate ( the gamma carbon of this side-chain ) and the amino group of the cysteine.