beta-galactosidase การใช้

• Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned
• No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page , except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ) , which is a selective marker of the wild - type virus . elisa test results suggested the expression of egf in cells , but not in culture supernant . the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract