cellulosome การใช้

• The high-affinity cohesin-dockerin interaction defines the cellulosome structure.
• "' Dockerin "'is a protein domain found in the cellulosome cellular structure of anaerobic bacteria.
• In contrast, all the " C . thermocellum " cellulosome catalytic components contain a single dockerin domain.
• Within a cellulosome, multiple endoglucanases, cellobiohydrolases, xylanases and other degradative enzymes work synergistically to attack heterogeneous, insoluble cellulose substrates.
• Other cellulosome systems ( such as those from " Acetivibrio cellulolyticus and Ruminococcus flavefaciens " ) appear to be even more intricate.
• Attachment of the cellulosome to its substrate is mediated by a scaffoldin-borne cellulose-binding module ( CBM ) that comprises part of the scaffoldin subunit.
• This interaction between the dockerin domains of the enzyme constituents of the cellulosome and the cohesin domains of the scaffoldin protein is essential to the construction of the cellulosome complex.
• This interaction between the dockerin domains of the enzyme constituents of the cellulosome and the cohesin domains of the scaffoldin protein is essential to the construction of the cellulosome complex.
• The scaffoldin component of the cellulolytic bacterium " Clostridium thermocellum " is a non-hydrolytic protein which organises the hydrolytic enzymes into a large complex, called the cellulosome.
• The cellulosome consists of a multi-functional integrating subunit ( called scaffoldin ), responsible for organizing the various cellulolytic subunits ( e . g ., the enzymes ) into the complex.
• Applications : Intelligent application of cellulosome hybrids and chimeric constructs ( " nanosomes " ) of cellulosomal domains should enable better use of cellulosic biomass and may offer a wide range of novel applications.
• More recently, anaerobic fungi, typified by " Piromyces equi ", have been suggested to also synthesise a cellulosome complex, although the dockerin sequences of the bacterial and fungal enzymes are completely different.
• The large size and heterogeneity of cellulosomes from the best-characterized organisms ( i . e ., " C . thermocellum, C . cellulolyticum, and C . cellulovorans " ) have greatly complicated efforts to probe cellulosome structure and function.