cycloheximide การใช้

• It can be considered as the acetylated analogue of cycloheximide.
• It is a structural component of cycloheximide, a very potent inhibitor of protein synthesis.
• These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin.
• The translational elongation freezing properties of cycloheximide are also used for ribosome profiling / translational profiling.
• CLS growth is essentially unaffected by antibiotic treatment with chloramphenicol, as well as by cycloheximide.
• The fungus has been selectively isolated using high temperature incubation ( at 40 癈 ) on media containing cycloheximide.
• Cycloheximide can be used as an experimental tool in molecular biology to determine the half-life of a protein.
• Cycloheximide treatment provides the ability to observe the half-life of a protein without confounding contributions from transcription or translation.
• Translation is halted via the addition of cycloheximide, and the DNA / RNA in the cell is then nuclease treated.
• Requiring a special medium, cycloheximide-treated McCoy or HeLa cells, and yields are still only 30-50 %.
• Growth has been observed in low oxygen environments . " Geomyces pannorum " is resistant to the antifungal agent cycloheximide.
• "' Cycloheximide "'is a eukaryote protein synthesis inhibitor, produced by the bacterium " Streptomyces griseus ".
• As previously mentioned, C . bertholletiae grow hyphally and reproduce dimorphic pathogenic fungi, growth of " C . bertholletiae " is inhibited by cycloheximide.
• The antibiotic cycloheximide is sometimes added to yeast growth media to inhibit the growth of " Saccharomyces " yeasts and select for wild / indigenous yeast species.
• Cycloheximide is frequently used in acute studies, because of its high level of inhibition and ease of reversibility ( Villers, Godaux, & Ris, 2012 ).
• Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis ( movement of two tRNA molecules and mRNA in relation to the ribosome ) thus blocking translational elongation.
• Treating cells with cycloheximide in a time-course experiment followed by Western blotting of the cell lysates for the protein of interest can show differences in protein half-life.
• Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied " in vitro " ( " i . e . " outside of organisms ).
• Because cycloheximide is degraded by alkaline conditions ( pH > 7 ), decontamination of work surfaces and containers can be achieved by washing with a non-harmful alkali solution such as soap.