fluorometric การใช้
- There are several fluorometric reagents for phosgene, and it can also be quantified with mass spectrometry.
- Fluorometric assays use a difference in the fluorescence of substrate from product to measure the enzyme reaction.
- It is used as choleretic and antispasmodic drugs and as a standard for the fluorometric determination of enzyme activity.
- It is also used traditionally as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.
- Using a detection antibody conjugated to an enzyme or fluorophore the quantity of bound protein can be accurately measured by fluorometric or colourimetric detection.
- For hydrolytic enzymes, colorimetric assays are required that use a p-nitrophenol ( p-NP )-linked substrate, or fluorometric assays that use a 4-methylumbelliferone ( MUF )-linked substrate.