immunoblotting การใช้

• They also discovered a sequence of human immunoblotting.
• This suggestion is supported by immunoblotting analyses indicating that the two are the same species.
• Using microarray and immunoblotting analyses it has been shown that miR-199a * targets the Met proto-oncogene.
• Because the proteins may retain or regain part of their structure during blotting they may react with specific antibodies giving rise to the term immunoblotting.
• BLV infection in humans can be identified by testing for antibodies against BLV using the immunoblotting and searching for antibodies against the purified recombinant p24 capsid protein.
• By applying SDS-polyacrylamide gel electrophoresis and subsequent immunoblotting, molecular weights of ? and ?-tubulins were determined to be 48 and 45 kDa, respectively.
• The six main antigens used in immunological laboratories for detection are RNP, Scl-70 and Jo1, which are screened for by Ouchterlony double immuno diffusion techniques and confirmed by immunoblotting.
• Cytoplasmic proteins and organelles are engulfed by autophagosomes which convert LC3-I to LC3-II . The presence of LC3-II serves as a marker for autophagy and can be detected by immunofluorescence or immunoblotting.
• In the biomedical sciences, PVDF is used in immunoblotting as an artificial membrane ( usually with 0.22 or 0.45 micrometre pore sizes ), on which proteins are transferred using electricity ( see western blotting ).
• :- The use of shRNA gene silencing combined with " immunoblotting " showed that BRAF inhibitors GDC-0879 and PLX4720 block MAPK signaling in BRAF ( V600E ) tumors while these same compounds activate the RAF-MEK-ERK pathway in KRAS mutant and RAS / RAF wild-type tumors.