intercalators การใช้

• Most intercalators are large polyaromatic compounds and are known or suspected carcinogens.
• DNA intercalators are used in chemotherapy to inhibit DNA replication in rapidly-growing cancer cells.
• This ability to bind to specific DNA base pairs allows for potential therapeutic applications of metallo-intercalators.
• Metallo-intercalators have a variety of potential therapeutic applications as a result of their structural diversity and universal photooxidative properties.
• This can be improved by using DNA stabilizing molecules such as intercalators like ortho-TINA, which will stabilize the duplex formation.
• Figure 2 : Metallo-intercalators enter double stranded DNA via the major groove and ?-stack between adjacent unbroken base pairs.
• "' DNA-binding metallo-intercalators "'are positively charged, planar, van der Waals, hydrophobic, electrostatic, and entropic interactions.
• In the case of ruthenium intercalators, the general synthesis consists of preparing intercalative ligands followed by their coupling to a ruthenium metal complex coordinated by specific ancillary ligands.
• As a result, DNA intercalators are often carcinogen s, with benzopyrene | benzopyrene diol epoxide, acridine s, aflatoxin and ethidium bromide being well-known examples.
• Interestingly, the lead compound is symmetrical whereas the target downstream secondary RNA structure is non-symmetrical, suggesting that both supposed intercalators are necessary for high-affinity binding.
• The information also gives vital clues to a molecule's mode of interaction with DNA . Molecules such as intercalators slot in between base pairs and interact through pi stacking.
• Figure 3 : The wide structure of metallo-intercalators containing the ligand 5, 6-chrysenequinone diimine ( chrysi ) can be used in anticancer therapeutics to identify mismatched DNA base pairs.
• Other chemicals, known as DNA intercalators, fit into the gap between adjacent bases on a single strand and induce frameshift mutations by " masquerading " as a base, causing the DNA replication machinery to skip or insert additional nucleotides at the intercalated site.
• One possible therapeutic application of metallo-intercalators is to combat cancerous tumor cells within the body by targeting specific mismatched DNA base pairs; the ability to modify the ligands bound to the metal center allows for a high degree of specificity in the binding interactions between the metallo-intercalator and the DNA sequence.