mekc การใช้
- MEKC has also been targeted for its potential to be used in combinatorial chemical analysis.
- MEKC can also be used in routine quality control of antibiotics in pharmaceuticals or feedstuffs.
- The sensitivity of conventional CEUV can be improved by using micellar electrokinetic chromatography ( MEKC ).
- In MEKC, however, techniques must be used to extend the elution range to separate strongly retained analytes.
- In most applications, MEKC is performed in open capillaries under alkaline conditions to generate a strong hydrophilic buffer solution as shown in " figure 1 ".
- Mixed micelle systems, such as the one formed by combining SDS with the neutral surfactant Brij-35, can also be used to alter the selectivity of MEKC.
- Recent applications of MEKC include the analysis of uncharged pesticides, essential and branched-chain amino acids in nutraceutical products, hydrocarbon and alcohol contents of the marjoram herb.
- Small sample and solvent requirements and the high resolving power of MEKC have enabled this technique to be used to quickly analyze a large number of compounds with good resolution.
- The extra term enclosed in parenthesis accounts for the partial mobility of the hydrophobic phase in MEKC . This equation resembles an expression derived for k ^ 1 in conventional packed bed chromatography:
- The basic set-up and detection methods used for MEKC are the same as those used in CE . The difference is that the solution contains a surfactant at a concentration that is greater than the critical micelle concentration ( CMC ).