microdissection การใช้

• It is the first developed laser microdissection system in Asia.
• More recent technologies utilize non-contact microdissection.
• Laser microdissection with mass spectrometry is the most reliable method of identifying the different forms of amyloidosis.
• Many approaches have been used in attempts to overcome this difficulty, including a variety of microdissection methods.
• Microdissection testicular sperm extraction in adult men with Klinefelter syndrome reported success rates of up to 45 %.
• Using a micromanipulator and microdissection, Seifriz showed that protoplasm was non-Newtonian, thixotropic and elastic.
• The technology merges laser capture microdissection ( LCM ) with micro array technology, to produce reverse phase protein microarrays.
• Back in the 1970s, modern microdissection techniques were brought to bear on the nerves and blood vessels that supply the penis.
• Chromosome microdissection is a specialized way of isolating these regions by removing the DNA from the band and making that DNA available for further study.
• In the laser microtome the laser-microdissection of internal areas in tissues, cellular structures, and other types of small features is also possible.
• Dr . Gordon and colleagues later combined laser capture microdissection, and functional genomics to characterize specified cell populations within the gastrointestinal tract, including multipotent stem cells.
• Prior to MALBAC, a single cell is isolated by various methods including laser capture microdissection, microfluidic devices, flow cytometry, or micro pipetting, then lysed.
• A fifth UV based technology uses standard glass slides coated with an inert energy transfer coating and a UV based laser microdissection system ( typically a Leica LMD or PALM Zeiss machine ).
• This led to development of laser capture microdissection ( LCM ), a technique that can isolate individual cell types individual cells, which gave rise to cDNA libraries of specific cell types.
• Another process follows gravity-assisted microdissection method that turns on gravity to collect samples in tube cap under the slide used ( used by ION LMD system, Jungwoo F & B ).
• Another closely related LCM process ( used by Leica ) cuts the sample from above and the sample drops via gravity ( gravity-assisted microdissection ) into a capture device below the sample.
• To prepare cells for chromosome microdissection, a scientist first treats them with a chemical that forces them into metaphase : a phase of the cell's life-cycle where the chromosomes are tightly coiled and highly visible.
• If a RPMA containing cell fraction lysates of a tissue / s is to be made, laser capture microdissection ( LCM ) or fine needle aspiration methods is used to isolate specific cells from a region of tissue microscopically.
• Unlike traditional methods that yield a mishmash of proteins, this " laser capture microdissection " isolates pure cells and, in seconds, transfers them to a special film that preserves the patterns proteins make as normal cells turn cancerous.
• Transport by gravity using an upright microscope ( called GAM, gravity-assisted microdissection ) or transport by " laser pressure catapult "; the most recent generation utilizes a technology based on laser induced forward transfer ( LIFT ).