preincubation การใช้

• 3 . amino acid assay found that besides the release of glu , preincubation with synaptosomes at 370c for 30 min enabled - agtx to reduce kcl induced asp , gly , gaba release from synaptosomes by 90 3 % , 94 . 5 2 % and 95 1 . 5 % , however ionomycin evoked release of these transmitters is not significantly affected . it is indicated - agtx has a common inhibitory effect on the release of these transmitters by a resemble mechanism
• Group division : experiments were performed in 3 different conditions , i . e . , normal 4 medium , high k * stimulated medium , and ca2 + - free medium . hippocampal slices from a same rat were then divided into 3 treatment groups : control group ? lices were oxygenated with 95 % o2 / 5 % cc > 2 and incubated at 35 ~ 36 ? for 30min ; hypoxia group ? lices were oxygenated and incubated on the same conditions as the control group , then the mixture gases change into 95 % o2 / 5 % co2 - hypoxic condition were remained for lomin ; ginkgolide b preincubation group ? lices will be preincubated at the normal oxygenated medium containing loomm ginkgolide b , 10 min later , repeated hypoxic treatment