puromycin การใช้

• Also of note, puromycin is critical in mRNA display.
• Selected hairpins are cloned into the resistance genes against ampicillin and puromycin.
• Plates containing puromycin are stable for 1 month when stored at 4 癈.
• Deletion of the gene encoding the multidrug efflux pump Pdr5 sensitizes cells to puromycin.
• All mRNA templates used for mRNA display technology have puromycin at their 3 end.
• These include anisomycin, cycloheximide, chloramphenicol, tetracycline, streptomycin, erythromycin, and puromycin.
• As a result, puromycin interferes with translation, and causes premature release of translation products.
• This enzyme participates in puromycin biosynthesis.
• Puromycin-resistant transformants are selected in LB agar medium supplemented with 125 礸 / ml of puromycin.
• Puromycin-resistant transformants are selected in LB agar medium supplemented with 125 礸 / ml of puromycin.
• "' Puromycin "'is an antibiotic that is a protein synthesis inhibitor by inhibiting translation.
• Lethal concentrations of puromycin are much higher for strains of " Saccharomyces cerevisiae " than mammalian cell lines.
• Compared to the cleavable ester bond in a tyrosyl-tRNA, puromycin has a non-hydrolysable amide bond.
• In contrast, the puromycin DNA spacer linker used in mRNA display technology is much smaller comparing to a ribosome.
• In this reaction, a puromycin molecule is chemically attached to the end of an mRNA template, which is then translated into protein.
• The puromycin can then form a covalent link to the growing peptide chain allowing the mRNA to be physically linked to its translational product.
• When the ribosome reaches the 3 end of the template, the fused puromycin will enter the A site of the ribosome . b.
• The ligated mRNA-DNA-puromycin library is translated in Red Nova Lysate ( Novagen ) or " E . Coli"
• This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate.