replicons การใช้

• Eukaryotes have multiple replicons, each with an " ori ".
• The Sindbis virus and Semliki Forest virus have been used to build recombinant alphavirus replicons.
• At " T ", both the replicons merge to complete the process of replication.
• This clockwise and counter-clockwise movement of two replicons is termed as bidirectional replication.
• The replicons range from 40 kb length, in yeast and " Drosophila ", to 300 kb in plants.
• She found that some resistance genes, including those for gentamycin resistance, are located on transposons and are readily transferred between replicons.
• These sub-viral replicons are small, non-coding, deletion mutants of the viral genome that maintain cis-acting RNA elements necessary for replication
• VCD115 is composed of four replicons : a main chromosome ( 2.82 Mb ) and three plasmids, P1 ( 325 kb ), P2 ( 314 kb ) and P3 ( 396 kb ).
• NS1 initiates and drives the viral  rolling hairpin replication mechanism ( RHR ), which is a linear adaptation of the more-common  rolling-circle replication strategy used by many small circular prokaryotic and viral replicons.
• It is not clear how alphavirus replicons raise an immune response, but it may be due to the high levels of protein expressed by this vector, replicon-induced cytokine responses, or replicon-induced apoptosis leading to enhanced antigen uptake by dendritic cells.
• If a sequence contains an inverted repeat ( a palindrome ) longer than 10 bp, and a spacer / insert of less than 75-150 basepairs, the sequence could be accessible to SbcCD, a protein which inhibits the propagation of replicons containing long palindromic DNA sequences.