ribonucleases การใช้

• Ribonucleases ( RNases ) degrade RNA and are involved in the RNA maturation process.
• The general catalytic mechanism used by ribozymes is similar to the mechanism used by protein ribonucleases.
• The sequence of pancreatic ribonucleases contains four conserved disulfide bonds and three amino acid residues involved in the catalytic activity.
• This gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily.
• This mode of catalysis is similar to how ribonucleases ( proteins ) function rather than any known small self-cleaving ribozyme.
• Pancreatic ribonucleases that are not inhibited by RI are approximately as toxic as alpha-sarcin, diphtheria toxin, or ricin.
• Holley's team of researchers determined the tRNA's structure by using two ribonucleases to split the tRNA molecule into pieces.
• He worked on other proteins as well, in particular ribonucleases, which were popular model systems for their small size and ease of crystallization.
• The team also found that the urine of pregnant women contains another type of protein, called ribonucleases, that destroys the genetic material in the HIV virus.
• First, direct probing via ribonucleases was performed and the subsequent modeling via mfold 3.0 using constraints from the probing results agrees with the proposed structure.
• Mammalian cells are protected from these effects due to their extremely high affinity for ribonuclease inhibitor ( RI ), which protects cellular RNA from degradation by pancreatic ribonucleases.
• Two pancreatic ribonucleases isolated from the oocytes of the Northern leopard frog-amphinase and ranpirnase-are not inhibited by RI and show differential cytotoxicity against tumor cells.
• Examples include ribonucleoside 2', 3'- cyclic monophosphates which are isolatable intermediates, and ribonucleoside 3'- monophosphates which are end products of the hydrolysis of RNA by certain ribonucleases.
• These repeats are found in a variety of non-homologous proteins, including bacterial ribonucleases, sulphite oxidases, reelin, netrins, sialidases, neuraminidases, some lipoprotein receptors, and a variety of glycosyl hydrolases.
• Ribonucleases can be divided into endoribonucleases and exoribonucleases, and comprise several sub-classes within the EC 2.7 ( for the phosphorolytic enzymes ) and 3.1 ( for the hydrolytic enzymes ) classes of enzymes.
• Lee-Huang said she and her group purified HCG and found it had no effect on HIV . The researchers then spent two years isolating other proteins in urine and testing them against HIV . Eventually they found lysozyme and ribonucleases.
• Ribonucleases ( or RNases ) are enzymes that DEAE cellulose as the adsorbent; it has a pH optimum of 4.5, lower than all other species; and, it has a " distinctly different " N-terminal amino acid sequence.
• The stability of mRNAs may be controlled by the 5'UTR and / or 3'UTR due to varying affinity for RNA degrading enzymes called ribonucleases and for ancillary proteins that can promote or inhibit RNA degradation . ( See also, C-rich stability element .)
• In prokaryotes, the initial mRNA transcript naturally possesses a 5'- triphosphate group after bacterial transcription; the enzyme RppH removes a pyrophosphate molecule from the 5'end, converting the 5'- triphosphate to a 5'- monophosphate, triggering mRNA degradation by ribonucleases.
• Hydroxymethylfurfural, which occurs naturally in several foods, is not associated with serious health risks . " P . indusiatus " also contains a unique ribonuclease ( an enzyme that cuts RNA into smaller components ) possessing several biochemical characteristics that differentiate it from other known mushroom ribonucleases.