rtpcr การใช้

• A complete cdna of 1047 bp was obtained by means of 5 ' - race ( 5 ' - rapid amplification of cdna end ) techniques using gene specific primer p1a5 - 1
• Furthermore , we found the mutagenesis relies on the alteration on gene expression profile induced by mnng treatment ; and also , the expression of dna polymerase p ( pol p ) was proved increased after mnng treatment
• Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts . the aberrant expression of sh2a gene in some cancers was found . it suggests that sh2a gene relates to tumors
• Normal tissues and tumor tissues methods a gene was cloned by exon trapping and exon linking technique . homologous analysis of the gene was researched by blast . then we explored the gene expression pattern in various human tissues by northern blot and rt - pcr
• Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) . the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l . in contrast to that of 850 bp from brassica napus l .
• This suggests that mxmybl represents a single copy gene in the genome of mains xiaojinensis . 8 expression pattern was analysed by northern blot and rt - pcr . the results showed that mxmybl mrna was expressed in root and leaf and it was strengthened expression by the treatment of fe - deficiency for three days in roots especially
• The n - terminal nucleotides 47 - 420 of the guangxi apmv - 1 isolates of different poultry species origin were amplified and sequenced . the alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of f gene of the guangxi isolates and other reference strains obtained from genbank were done
• The results of gst - pulldown and yeast two - hybrid showed kyot2 interact with them respectively in and yeast and vitro . 3 using the yeast two - hybrid system , we isolated a novel protein , kbp ( kyot binding protein ) . three types of cdnas were identified by rt - pcr and were designated as kbp1 , kbp2 , kbp3 , respectively
• According to the gene sequence and secondary structure of hcv ns5b , we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et . al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion , the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
• The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr . sequence analysis of the gene was described in this papel the amino acid sequence , as derived from the nucleotide sequence of a cdna clone , had high homology with other subtilisin - like serine protease of nematogenous fimgi
• But the primers designed for vvibdv can only amplify vvibdv strains . so we can typing strains between vvibdv and cibdv by using vvibdv - specific primers by one reaction . typing the pathotype of field ibdv in guangxi , 23 field sample " fragments were amplified by the primary rt - pcr or nested - pcr . they were cleaved with sspl and sacl