supercoiled การใช้

• As a general rule, the DNA of most organisms is negatively supercoiled.
• The viral DNA forms supercoiled mini-chromosome structures upon entering the host nucleus.
• DNA of most organisms is negatively supercoiled.
• The circular supercoiled plasmids'strands will stay relatively closely aligned and will renature correctly.
• Supercoiled DNA forms two structures; a plectoneme or a toroid, or a combination of both.
• Therefore, the genomic DNA will form an insoluble aggregate and the supercoiled plasmids will be left in solution.
• Gels of plasmid preparations usually show a major band of supercoiled DNA with other fainter bands in the same lane.
• As replication occurs, DNA ahead of the replication bubble becomes positively supercoiled, while DNA behind the replication fork becomes entangled forming precatenanes.
• It also inhibits the nicking-closing activity on the subunit of DNA gyrase that releases the positive binding stress on the supercoiled DNA.
• A DNA segment with excess or insufficient helical twisting is referred to, respectively, as positively or negatively " supercoiled ".
• In 2008 it was noted that transcription twists DNA, leaving a trail of undercoiled ( or negatively supercoiled ) DNA in its wake.
• Circular DNA such as plasmids, however, may show multiple bands, the speed of migration may depend on whether it is relaxed or supercoiled.
• The movement of the DNA may be affected by the supercoiled DNA usually moves faster than relaxed DNA because it is tightly coiled and hence more compact.
• After entering the host cell, these single stranded " nicks " in the viral DNA are repaired, forming a supercoiled molecule that binds to histones.
• The word " transfection " is a supercoiled plasmid DNA or siRNA constructs ), or even proteins such as antibodies, may be transfected.
• An old standing dynamic problem is how DNA " self-replication " takes place in living cells that should involve transient uncoiling of supercoiled DNA fibers.
• A procedure that has first been derived by Levitt for the description of protein folding and later been used for supercoiled DNA by Klenin and Langowski is to compute
• DNA " in vivo " is typically negatively supercoiled, which facilitates the unwinding ( melting ) of the double-helix required for RNA transcription.
• Positively supercoiled ( overwound ) DNA is transiently generated during DNA replication and transcription, and, if not promptly relaxed, inhibits ( regulates ) these processes.
• Cells embedded in agarose on a microscope slide are lysed with detergent and high salt to form nucleoids containing supercoiled loops of DNA linked to the nuclear matrix.