supernate การใช้

• The supemate of cell culture was collected 48 hours after transfection . this supernate was also called conditional media
• The supernate of virus culture was used as templet in overlapping pcr , then the interesting gene was obtained
• Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid , and induced with methanol to secrete interesting peptide . the supernate of pichia pastoris culture was analysed by sds - page and western blotting . a reactive band , which the apparent molecular weight is 36kd , can be detected with sheep anti - hcmv polyclonal antibodies