supravital การใช้

• Those that enter and stain living cells are called supravital stains ( e . g.
• "' Brilliant cresyl blue "'is a supravital stain used for counting reticulocytes.
• Together with Janus Green B, it is used to stain embryonal tissues and supravital staining of blood.
• Hoechst dyes are cell-supravital ", meaning that live cells survive a treatment with these compounds.
• Supravital staining can be combined with cell surface antibody staining ( immunofluorescence ) for applications such as FACS analysis.
• A traditional application of methylene blue is the intravital or supravital staining of nerve fibers, an effect first described by Paul Ehrlich in 1887.
• Although antibodies may be used in a similar way in this context, the term " supravital stain " is typically reserved for smaller chemicals which possess suitable properties intrinsically.
• The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes it possible to see the reticulofilamentous pattern of ribosomes characteristically precipitated in these live immature red blood cells by the supravital stains.
• The most common supravital stain is performed on reticulocytes using new methylene blue or brilliant cresyl blue, which makes it possible to see the reticulofilamentous pattern of ribosomes characteristically precipitated in these live immature red blood cells by the supravital stains.
• Partly due to their toxic interaction inside a living cell, when supravital stains enter a living cell, they might produce a characteristic pattern of staining different from the staining of an already fixed cell ( e . g . " reticulocyte " look versus diffuse " polychromasia " ).
• Besides his role in the formulation of the famous Michaelis Menten equation ( 1913 ) he discovered Janus green as a supravital stain for mitochondria and the Michaelis Gutmann body in urinary tract infections ( 1902 ) and found that thioglycolic acid could dissolve keratin, a discovery that would come to have several implications in the cosmetic industry, including the permanent wave.