thioester การใช้

• The intermediate that attacks the incoming thioester substrate.
• The challenge lies in the preparation of the necessary unprotected peptide-thioester building block.
• This enzyme belongs to the family of hydrolases, specifically the class of thioester lyases.
• This enzyme belongs to the family of hydrolases, specifically those acting on thioester bonds.
• First, a prenyl lipid anchor is attached to the cysteine through a thioester linkage.
• Upon cleavage of the LPXTG motif, Sortase forms a thioester intermediate with the engineered molecule.
• The absence of this domain means that the exposed thioester bond of active TEP1 is unstable.
• They use the coenzyme A-thioester of ( r )-hydroxy fatty acids as substrates.
• This highly stereoselective chiral inversion is mediated by the FLX-S-Acyl-CoA thioester.
• During the second step, Atg8 is transferred to Atg3 assuming the same type of thioester bondage.
• The energy needed for this oxidation is conserved in the formation of a thioester bond of succinyl CoA.
• Deprotonation encourages the reformation of the carbonyl group in the thioester intermediate and ejection of the hydride ion.
• Although this secondary ubiquitin is similarly adenylated, it does not form the same thioester complex described previously.
• Cleavage into the cut form is followed by a change in protein structure which exposes the thioester bond.
• Finally, in the extein the ester or thioester bond is rearranged to form a normal peptide bond.
• Similarly, a recombinant protein containing an N-terminal Cys can be reacted with a synthetic polypeptide thioester.
• The most common form of native chemical ligation uses a peptide thioester that reacts with a terminal cysteine residue.
• The energy from ATP and diphosphate hydrolysis drives the formation of this reactive thioester, and subsequent steps are thermoneutral.
• "' Methylmalonyl-CoA "'is the thioester consisting of coenzyme A linked to methylmalonic acid.
• The thiolase superfamily enzymes catalyse the carbon carbon-bond formation via a thioester-dependent Claisen condensation reaction mechanism.