tlck การใช้

• The po is very sensitive to some trypsin - specific inhibitors such as sbti , p - apmsf and bestatin , but almost not affected by trypsin - specific inhibitor tlck at all , indicates that it is like a tyrosine - type protease
• The he was strongly inhibited by sbti and apmsf , and very sensitive to pmsf , lbti , and tlck , while not sensitive to chymostatin , bestatin , leupeptin , tpck , pepstatin , nem and iam . all these results imply that brine shrimp he was most probably a trypsin - type serine protease . the he could be strongly inhibited by edta in a dose - dependent manner , and 50 mmol / l edta exhibited more than 56 . 5 % inhibition
• Purified yntatl trypanosomes were broken by n - tosyl - lysine chloromethyl ketone ( tlck ) and centrifugated by high g to form soluble proteins . the soluble proteins were chromatographed on a de - 52 column . the material in the first peak constituted the purified vsg
• Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7 . 5 , 250mm nacl , 5mm edta , imm dtt , 0 . 1 % triton , 50mm sodium orthovanadate , looug / mg pmsf and tpck , 50ug / ml tlck , 1 ug / ml leupeptin pepstatin and aprotinin ) , frozing them below - 70 , and then thawing them at room temperature for 10 min