topoisomerase การใช้

• Topoisomerase IIA plays an essential role in resolving these topological problems.
• Topoisomerase I and SR proteins also interact to increase genome stabilization.
• They act as anti-cancer drugs by inhibiting topoisomerase II.
• Some organisms have two isoforms of topoisomerase II : alpha and beta.
• Topoisomerase poisons have been extensively used as both anticancer and antibacterial therapies.
• Cadmium cations react with the topoisomerase in the following manner:
• The topo I of both prokaryotes and eukaryotes are the type I topoisomerase.
• CcdB protein is a topoisomerase poison from " Escherichia coli ".
• Topoisomerase inhibitors can also function as antibacterial agents.
• Also, cells deficient in topoisomerase II show significantly reduced Aurora B kinase activity.
• The introduction of DSBs in DNA often employs the topoisomerase-like protein SPO11.
• It is believed that eukaryotic cells do not contain DNA gyrase or topoisomerase IV.
• However, when Topoisomerase IV alone was inhibited, decatenation was almost completely blocked.
• Cadmium has been shown to be carcinogenic due to interactions with DNA topoisomerase II?.
• It works by blocking the topoisomerase enzyme that is essential for cancer cells to proliferate.
• Gyrase ( a form of topoisomerase ) relaxes and undoes the supercoiling caused by helicase.
• Topoisomerase 1 inhibition is synthetically lethal with deficiency of expression of certain DNA repair genes.
• In cancers, the topoisomerase II-alpha is highly expressed in highly proliferating cells.
• Both gyrase and topoisomerase IV CTDs bend DNA, but only gyrase introduces negative supercoils.
• It also shows an antileishmanial activity in animal models being an inhibitor of topoisomerase I.