eif4f การใช้

• Leucine indirectly activates eIF4F complex, which are essential for mRNA binding in translational initiation.
• In mammals, eIF4F is bridged to the 40S ribosomal subunit by eIF3 via eIF4G, while budding yeast lacks this connection.
• Once in the cytoplasm after the pioneer round of translation, the CBC is replaced by the translation factors eIF4E and eIF4G of the eIF4F complex.
• The eIF4F proteins interact with a number of different binding partners, and there are multiple genetic isoforms of eIF4A, eIF4E, and eIF4G in the human genome.
• In the case of BTE it " tricks " eIF4F ( eIF4E, eIF4G are parts of eIF4F ) into " telling " the ribosome that a 7mG cap is present.
• In the case of BTE it " tricks " eIF4F ( eIF4E, eIF4G are parts of eIF4F ) into " telling " the ribosome that a 7mG cap is present.
• The Poly ( A )-binding protein ( PABP ) also associates with the eIF4F complex via eIF4G, and binds the poly-A tail of most eukaryotic mRNA molecules.
• The eIF4F complex is composed of three subunits : eIF4A, eIF4E, and eIF4G . Each subunit has multiple human isoforms and there exist additional eIF4 proteins : eIF4B and eIF4H.
• The other subunits of eIF4F are a 47-kD polypeptide, termed eIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD scaffolding polypeptide, eIF4G.
• Three isoforms of eIF4A exist; I and II share 95 % amino acid similarity and have been found simultaneously in rabbit reticulocyte eIF4F in a ratio of 4 : 1, respectively.
• The IRES achieves this by mediating the internal initiation of translation by recruiting a ribosomal 43S pre-initiation complex directly to the initiation codon and eliminates the requirement for the eukaryotic initiation factor eIF4F.
• Alternative splicing results in five transcript variants encoding four distinct isoforms . eIF4G serves as a scaffold, interacting with mRNA and the other components of the eIF4F complex, as well as the PABP and eIF3.
• EIF4G is a component of the eIF4F complex, containing eIF4E, another initiation factor bound to the 5'cap on the 5'end of mRNA . This binding forms the characteristic loop structure of deadenylation and, ultimately, turnover.
• The protein encoded by this gene is a component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'- terminal secondary structure, and recruitment of mRNA to the ribosome.
• Cap-dependent translation involves a series of steps that join the small and large ribosomal subunits at the start codon of mRNA . The initiation factor complex eIF4F is dependent upon the presence of a 5 mRNA cap upstream from the start codon in order to initiate translation . 4
• The IRES achieves this by mediating the internal initiation of translation by recruiting a ribosomal 43S pre-initiation complex directly to the initiation codon and eliminates the requirement for the eukaryotic initiation factor, eIF4F . The classical swine fever virus UTR described appears to be longer at the 5'end than other pestivirus UTRs.
• After the first round of translation ( " pioneer round " ), CBC20 / 80 is replaced by the translation initiation factor eIF4E . The eIF4F complex ( eIF4E, eIF4G and eIF4A ) then regulates translation in response to the state of the cell via its phosphorylation state and again protects the message from decapping.
• The "'Tobamovirus internal ribosome entry site "'( IRES ) is an element that allows cap and end-independent IRES achieves this by mediating the internal initiation of translation by recruiting a ribosomal 43S pre-initiation complex directly to the initiation codon and eliminates the requirement for the eukaryotic initiation factor, eIF4F.
• Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F ( eIF4F ), which is a cap binding protein complex that consists of three subunits : eIF4A, eIF4E and eIF4G . The protein encoded by the eIF4G2 gene shares similarity with the C-terminal region of eIF4G1 that contains the binding sites for eIF4A and eIF3 . eIF4G2 additionally contains a binding site for eIF4E at the N-terminus.
• EIF4G is a 175.5-kDa scaffolding protein that interacts with eIF3 and the Poly ( A )-binding protein ( PABP ), as well as the other members of the eIF4F complex . eIF4E recognizes and binds to the 5'cap structure of mRNA, while eIF4G binds PABP, which binds the poly ( A ) tail, potentially circularizing and activating the bound mRNA . eIF4Aa DEAD box RNA helicaseis important for resolving mRNA secondary structures.