eif4g การใช้

• Some viral proteases cleave off part of eIF4G, that contains the eIF4E binding region.
• Second, the CBC and eIF4E / eIF4G block the access of decapping enzymes to the cap.
• PABP-C1 evicted from eIF4G by NSP3 accumulates in the nucleus of rotavirus-infected cells.
• The virus may even use partially-cleaved eIF4G to aid in initiation of IRES-mediated translation.
• BTE has been found to bind to eIF4G and weakly to eIF4E ( proteins involved in translation initiation ).
• Unlike other picornaviruses, this virus requires an intact eukaryote initiating factor 4G ( eIF4G ) for the initiation of translation.
• Across species, eIF4G strongly associates with the protein that directly binds the mRNA cap : eIF4E . The eIF4A ).
• In mammals, eIF4F is bridged to the 40S ribosomal subunit by eIF3 via eIF4G, while budding yeast lacks this connection.
• The cytosolic isoform of eukaryotes'Poly ( A ) binding protein binds to the initiation factor eIF4G via its C-terminal domain.
• Another regulator is 4EBP, which binds to the initiation factor eIF4E and inhibits its interactions with eIF4G, thus preventing cap-dependent initiation.
• Site-directed mutagenesis and isothermal titration calorimetry documented that NSP3 and PABP use analogous eIF4G recognition strategies, despite marked differences in tertiary structure.
• One example of a, eIF4G binding site in a viral IRES's is in the EMCV IRES ( nucleotides 746-949 ).
• Once in the cytoplasm after the pioneer round of translation, the CBC is replaced by the translation factors eIF4E and eIF4G of the eIF4F complex.
• The FMRP / CYFIP1 complex binds in such a way as to prevent the eIF4E-eIF4G interaction, which is necessary for translation to occur.
• This has the effect of preventing most cellular mRNAs from binding eIF4G; however, a few cellular mRNAs with IRESs still translate under these conditions.
• Natural alleles of host translation elongation initiation factors " eif4e " and " eif4g " are also recessive viral-resistance genes.
• Proteins that contain MIF4G ( middle of eIF4G ( MIM 600495 ) ) and / or MA3 domains, such as NOM1, function in protein translation.
• The eIF4F proteins interact with a number of different binding partners, and there are multiple genetic isoforms of eIF4A, eIF4E, and eIF4G in the human genome.
• Within the cell eIF4G is found primarily in the cytoplasm, usually bound to eIF4E; however, it is also found in the nucleus where its function is unknown.
• In the case of BTE it " tricks " eIF4F ( eIF4E, eIF4G are parts of eIF4F ) into " telling " the ribosome that a 7mG cap is present.