gm-csf การใช้

• Anti - lymphoma immunity induced by el - 4 cells transfected with b7 . 1or and gm - csf gene
• Effects of carrying murine gm - csf gene adenovirus vector on immune response of b16 melanoma bearing mice
• 5 the expression of gm - csf mrna in the nscs was detected by in situ hybridization
• Construction of a plasmid co - expressing eastern equine encephalitis virus e2 gene and mouse gm - csf gene and its immune effects
• Granulocyte - macrophage colony - stimulating factor ( gm - csf ) exists widely in the hematopoietic system , immune system and nervous system
• Evidences showed that it contributed to proliferation and differentiation of many kinds of cells , and gm - csf also improved the livability of neuron in vitro and reduced its apoptosis rate
• Indeed , although gm - csf - stirnulated b7 - deficient dc progenitors induced only low levels of t cell priining and prolonged allograft survival , they failed to induce indefmite cardiac allogr8ft sed
• But the understanding of effects of gm - csf on nscs is still poor . experimental precedure 1 immunohistochemistry was applied to detect nestin - positive cells in dentate gyrus of hippocampus of rats at the first day after birth
• The accine was constructed of lethally irradiated pancreatic cancer cells , engineered to include a granulocyte macrophage colony stimulating factor ( gm - csf ) gene that recruits immune - system cells to seek and destroy microscopic cancers
• Jak2 is one of the janus kinase family . it participates in the jak - stat signal transduction and plays roles in signal transduction of many cytokins and growth factors such as growth hormone , il and gm - csf . it plays essential roles in cellular functions , i . e . cell proliferation and differentiation
• Immature dc generated from mouse bone marrow by culturing in gm - csf and il - 4 for 5 days were incubated with hs - exo or exo for 48 hours , and then were collected for phenotypical analysis and phagocytosis of ova - fitc by facs technique . cytokines in the culture supernatants were quantitated using elisa . in the experiment of mlr to detect
• On the other hand , there is substanial evidences tha dcs mediate tolerane . the gm - csf - stbolated mouse bone mwderived mhc class ii + dc progendors tha are deficient in cell surface erpression of the costbolatory molecules b7 - l and b7 - 2 can induce alloanginspecific t ceil anergy in vbo . systendc adchstraion of these donor - derived dc progendors to recipients intravenously 7 days before transplantation prolonged the median graft survival tane from 9 . 5 days to 22 days wbout additional twnosuppressions in a mouse cardinc twlanation model
• Using of g - csf / gm - csf can mobilize the cec / cep . the amount of cecs / ceps is correlated with the serum level of vegf > vcam - k 1cam - 1 > bfgf , which indicate that these cytokines are related to cec / cep mobilization . the ceps can differentiate into mature endothelial cells in vitro which is testified by electron microscope and immunohistochemistry
• Cd 14 * monocytes or bone marrow derived hematopoietic progenitor cells cultured with granulocyte - macrophage colony - stimulating factor ( gm - csf ) and il - 4 differentiate into efficient apc with morphology and cell surface molecule expression typical of immature dc . it was demonstrated that a variety of factors ( such as lps , tnf - a , il - 1 , monocyte - conditioned medium , and cd40 receptor cross - linking ) induce the maturation of immature dc to mature dc that are much more potent in activating t cells by increasing the expression of costimulatory molecules and cytokines , promoting the migration of dc to draining lymph nodes , and down - regulating the capacity of dc to capture and process antigen