igf-i การใช้

• Effect of cysteamine on the plasma contents of gh , ss and igf - i in adult geese
• Igf - i , insulin - like growth factor
• The e . coli bl21 ( de3 ) was transformed with the constructed recombinant plasmid pexsecl - igf - i
• We modified it with a synthetic gene that would produce igf - i only in skeletal muscle and began by trying it out in normal mice
• So it is very helpful to advance the research and application of igf - i to establish a relatively simple proficient efficient purification route
• We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme . the product of pet - 32a ( + ) - igf - i was analyzed by western blotting , which confirmed that the hybrid protein was expressed as expected
• Insulin - like growth factor - i ( igf - i ) is a kind of active peptide that can promote many types of cells to grow . it plays an important regulatory role in the proliferation , differentiation , apoptosis of tissue cell and tumorigeneisis
• Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3
• Research , japan . there dr . jing helped to set up a primary culture system of neuroepithelial cells of day 10 mouse embryos . then he found a human teratocarcinoma cell line , pa - 1 , secreted the growth factors related to egf and igf - i
• Sds - page analysis suggested that the bacteria containing the recombinant plasmid pet - 32a ( + ) - igf - i produced the fusion protein of 30kda as it was induced by iptg . consisting 10 % of the total bacterial proteins , and the pet - 30a ( + ) - igf - ii produced the fusion protein of 14kda , which consisting 35 % of total bacterial proteins . 5
• 4 ) either low - sugar dmem , tcm199 , dmem + f12 ( 1 : 1 ) support qinchuan - scalper skin fibroblast cells growth well , but low - sugar dmem caused best effect 5 ) eof and igf - i added in low - sugar dmem could promote qinchuan - scalper skin fibroblast cells growth potent respectively , yet they have cooperated effect each other