oligoribonucleotides การใช้

• Upon the addition of 2-5A molecules, RNase L becomes active, cleaving the oligoribonucleotides and interfering in the FRET signal.
• Four nucleotides are the minimal requirement for RNA recognition by rotavirus non-structural protein NSP3 : using short oligoribonucleotides, it was established that the minimal RNA sequence required for binding of NSP3A is "'GACC " '.
• This Fluorescence Resonance Energy Transfer ( FRET ), as oligoribonucleotides containing a quencher and a fluorophore on opposite sites are added to a solution with inactive RNase L . The FRET signal is then recorded as the quencher and the fluorophore are very close to each other.
• This Fluorescence Resonance Energy Transfer ( FRET ), as a oligoribonucleotides containing a quencher and a fluorophore on opposite sites are added to a solution with inactive RNase L . The FRET signal is then recorded as the quencher and the fluorophore are very close to each other.