tfiia การใช้

• Genetic analysis in yeast has shown that TFIIA is essential for viability.
• TFIIA is a heterodimer with two conserved.
• A second gene encoding a large TFIIA subunit has been found in some higher eukaryotes.
• TFIIA interacts with the activators, assisting with their ability to increase, or activate, transcription.
• The minimal PIC includes RNA polymerase II and six general transcription factors : TFIIA, chromatin remodeling complexes may also be components of the PIC.
• "' Transcription factor TFIIA "'is a preinitiation complex ( PIC ) capable of initiating RNA synthesis from a DNA template.
• The requirement for TFIIA " in vitro " transcription systems has been variable, and it can be considered either as a GTF and / or a loosely associated TAF-like coactivator.
• This gene, ALF / TFIIAtau ( gene name GTF2A1LF ) is expressed only in oocytes and spermatocytes, suggesting it has a TFIIA-like regulatory role for gene expression only in germ cells.
• Some of the other recruited transcription factors include TFIIA, TFIIB, and TFIIF . Each of these transcription factors is formed from the interaction of many protein subunits, indicating that transcription is a heavily regulated process.
• The TATA box is usually found at the binding site of RNA polymerase II . TFIID, a transcription factor, binds to the TATA box, followed by TFIIA binding to the upstream part of the TFIID protein.
• The most common GTFs are TFIIA, TFIIB, TFIID ( see also TATA binding protein ), TFIIE, TFIIF, and TFIIH . The preinitiation complex binds to promoter regions of DNA upstream to the gene that they regulate.
• TFIIB then binds to the " downstream " part of TFIID . RNA polymerase can then recognize this multi-protein complex and bind to it, along with various other transcription factors such as TFIIF, TFIIE and TFIIH . Transcription is then initiated, and the polymerase moves along the DNA strand, leaving TFIID and TFIIA bound to the TATA box.