cytometric การใช้

• Flow cytometric separation of X and Y spermatozoa for the prevention of X-linked diseases.
• Flow cytometric analysis showed that p53 expression induces ROS production, which increases as apoptosis progresses under some conditions.
• Dr . Johnson and colleagues reported the first successful flow cytometric separation of X and Y bearing human sperm into highly enriched populations.
• Flow cytometric assays have demonstrated comparable sensitivity to immunofluorescence tests, with the additional benefit of simultaneous detection of multiple autoantibodies relevant to vasculitis.
• Prior to flow cytometric sorting, semen is labeled with a fluorescent dye called Hoechst 33342 which binds to the DNA of each spermatozoon.
• Several FSL constructs have been used to label viruses to assist in their flow-cytometric visualisation and to track them real time distribution in animal models.
• Finally, cytometric fingerprinting uses a variant of frequency difference gating to set bins and measure for a series of samples how many cells fall within each bin.
• An Flow cytometric analysis of eosin-52-maleimide-labeled intact red blood cells and the acidified glycerol lysis test are two additional options to aid diagnosis.
• 12.2013 " Changes of Th1 / Th2 / Th17 in patients received non-myeloablative haploidentical hematopoietic stem cell transplantation detected by flow cytometric bead array"
• Successful flow cytometric sorting of sperm depends upon the accurate detection of differences in fluorescent signal intensity ( a function of sperm DNA content ) between the X and Y bearing sperm.
• Another cytometric technique used in sperm sorting is magnetic-activated cell sorting ( MACS ) which is routinely applied in assisted reproduction hospitals to sort out sperm with fragmented DNA . This is achieved using antibodies to surface markers of programmed cell death ( apoptosis ) such as annexin V, coupled with magnetic beads.
• His main contributions range from the characterization of the molecular causes of penicillin allergy, the suppression of allergic reactions using hapten inhibition, the international standardization of allergens to the study of Immunoglobulin E ( IgE ) synthesis and associated receptors, and the development of new diagnostics techniques such as in-vitro cellular assays as well as flow cytometric tests.
• To date, over 900 babies have been born after using MicroSort?for sorting fresh or frozen sperm into enriched X-and Y bearing populations for use in IUI, IVF and ICSI . Flow cytometric sperm sorting in the livestock species and other animals was licensed by USDA to XY, Inc ., a company in Colorado, and has resulted in the birth of tens of thousands of offspring in North and South America, Europe and Asia.
• Furthermore, unlike for the pro-erythroblast and later stages of erythroid development, no truly reliable and unique positive flow-cytometric markers exist, though it is possible to use negative exclusion markers to deplete a cell population of other precursors and differentiated cells by cell sorting, thus greatly enriching it for the CFU-e activity ( Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit, Terszowski G . et al.