cytopathogenic การใช้

• Compared to its parental virus bartha - k61 strain , it displayed no obvious differences in virus multiplication and cytopathogenic effects in different cell cultures ( pk - 15 , ibrs2 , vero and cef )
• After obvious cytopathogenic effects developed , virus - contained supematants were harvested , and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal . single blue plaques were picked , and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification . the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
• The bvdv nadl strain is cytopathogenic . searching fromgenbank several cp strains " sequence of e2 gene about deer - n21 , sh9 , newyork - i and so on were found . according to the homologous sequence they were designed and synthesised a pair of primers by the biology software primer 5 and added bal i and nco i site to the 5 " end which can be used for the application of e2 gene and subcloning