desmin การใช้

• In cardiac muscle, desmin is present in ?B-crystallin.
• The presence of desmin and myogenin can be detected using immunohistochemistry.
• Desmin is a 53.5 kD protein composed of 470 amino acids.
• Desmin is a subunit of intermediate filaments in cardiac muscle, skeletal muscle and smooth muscle tissue.
• Activated satellite cells also begin expressing muscle-specific filament proteins such as desmin as they differentiate.
• Desmin has been evaluated for role in assessing the depth of invasion of urothelial carcinoma in TURBT specimens.
• Desmin is one of the earliest protein markers for muscle tissue in embryogenesis as it is detected in the somites.
• Later, a yeast two-hybrid method was used to demonstrate that syncoilin is a binding partner of desmin.
• LAM cells stain positively for smooth muscle actin, vimentin, desmin, and, often, estrogen and progesterone receptors.
• Desmin protein may also bind to the DAG complex, and regions of it are known to be involved in signaling.
• Staining is positive for vimentin, negative for cytokeratin and desmin, and variable for CD34, Factor VIIIa, S-100.
• It is classified by gene structure and coding sequence as a type III IF protein because of its homology with vimentin, GFAP and desmin.
• In adult muscle, desmin forms a scaffold around the Z-disk of the sarcomere and connects the Z-disk to the subsarcolemmal cytoskeleton.
• Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy.
• The condition now called Desmin-related myofibrillar myopathy ( also called myofibrillar myopathy-1 ) was formerly called inclusion body myopathy 1 ( IBM1 ).
• Diagnosis may be aided by immunostaining; endometrial stromal nodules are positive for CD10; leiomyomas are positive for caldesmon and desmin ( and sometimes CD10 ).
• A separate study examined this in more detail in cardiac tissue and found that murine hearts lacking desmin developed hypertrophic cardiomyopathy and chamber dilation combined with systolic dysfunction.
• There are three major domains to the desmin protein : a conserved alpha helix rod, a variable non alpha helix head, and a carboxy-terminal tail.
• Since desmin links the mitochondria to the sarcomere it may transmit information about contractions and energy need and through this regulate the aerobic respiration rate of the muscle cell.
• On immunohistochemistry, these cells have trilinear coexpression including the epithelial marker cytokeratin, the mesenchymal markers desmin and vimentin, and the neuronal marker neuron-specific enolase.